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mouse anti isl1  (Novus Biologicals)


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    Novus Biologicals mouse anti isl1
    Mouse Anti Isl1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+isl1/Islet-1+Antibody+(2E7)/pmc12844902-418-94-97
    Average 94 stars, based on 1 article reviews
    mouse anti isl1 - by Bioz Stars, 2026-10
    94/100 stars

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    Article Title: Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors
    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-ANK3 (ANKYRIN-G), 1:250 (Santa Cruz, sc-12719); rat anti-BrdU, 1:500 (AbD Serotec, OBT0030); rabbit anti-CSPG4 (NG2), 1:500 (Millipore, AB5320); rabbit anti-CTIP2, 1:500 (Abcam, ab28448); rat anti-CTIP2, 1:250 (Abcam, ab18465); rabbit anti-CUX1, 1:200 (Santa Cruz Biotechnology, sc-13024); rabbit anti-DARPP32, 1:250 (Cell Signaling Technology, 2306 S); rabbit anti-FOG2, 1:250 (Santa Cruz Biotechnology, sc-10755); rabbit anti-FOXP2, 1:2000 (Abcam, AB16064); mouse anti-GABA, 1:200 (Sigma, A0310); mouse anti-GFAP, 1:1000 (Sigma, G3893); rabbit anti-GFAP, 1:1000 (Sigma, G9269); chicken anti-GFP, 1:1000 (Invitrogen, A10262); rabbit anti-GFP, 1:1000 (Invitrogen, A11122); mouse anti-HA, 1:1000 (Covance, MMS-101R); mouse anti-ISL1, 1:250 (Novus, H00003670); mouse anti-MAP2, 1:500 (Sigma, M1406); chicken anti-NESTIN, 1:2000 (Novus, NB100-1604); mouse anti-NeuN, 1:500 (Chemicon, MAB377); rabbit anti-NF-M, 1:200 (Millipore, AB1987); mouse anti-NEUROG2, 1:100 (R&D Systems; MAB3314); goat anti-OLIG2, 1:200 (R&D Systems, AF2418); rat anti-RFP, 1:500 (antibodies-online, ABIN334653); rabbit anti-PCP4, 1:500 (Proteintech, 14705–1-AP); rabbit anti-PDGFRB, 1:100 (Cell Signaling, 3169); mouse anti-PSA-NCAM, 1:200 (Chemicon, MAB5324); mouse anti-SATB2, 1:200 (Abcam, ab51502); rabbit anti-SATB2, 1:500 (Abcam, ab34735); rabbit anti-SOX6, 1:500 (Abcam, AB30455); goat anti-SOX10, 1:200 (Santa Cruz, sc-17342); rabbit anti-SYNAPSIN, 1:500 (Synaptic Systems, 106002); mouse anti-SYNAPTOPHYSIN, 1:500 (Millipore, MAB5258); rabbit anti-TH, 1:250 (Millipore, AB152); rabbit anti-TUBB3 (TUJ1), 1:1000 (Sigma, T2200); mouse anti-TUBB3 (TUJ1), 1:1000 (Biolegend, MMS-435P), rabbit anti-vGLUT1, 1:500 (Synaptic Systems, 135302); rabbit anti-2A-peptide, 1:1000 (Millipore, ABS31), rabbit anti-5HT, 1:3000 (Immunostar, 20080).

    Article Title: Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors
    Article Snippet: The following primary antibodies and dilutions were used: mouse anti- ANK3 (ANKYRIN- G), 1:250 (Santa Cruz, sc- 12719); rat anti- BrdU, 1:500 (AbD Serotec, OBT0030); rabbit anti- CSPG4 (NG2), 1:500 (Millipore, AB5320); rabbit anti- CTIP2, 1:500 (Abcam, ab28448); rat anti- CTIP2, 1:250 (Abcam, ab18465); rabbit anti- CUX1, 1:200 (Santa Cruz Biotechnology, sc- 13024); rabbit anti- DARPP32, 1:250 (Cell Signaling Technology, 2306 S); rabbit anti- FOG2, 1:250 (Santa Cruz Biotechnology, sc- 10755); rabbit anti- FOXP2, 1:2000 (Abcam, AB16064); mouse anti- GABA, 1:200 (Sigma, A0310); mouse antiGFAP, 1:1000 (Sigma, G3893); rabbit anti- GFAP, 1:1000 (Sigma, G9269); chicken anti- GFP, 1:1000 (Invitrogen, A10262); rabbit anti- GFP, 1:1000 (Invitrogen, A11122); mouse anti- HA, 1:1000 (Covance, MMS- 101R); mouse anti- ISL1, 1:250 (Novus, H00003670); mouse anti- MAP2, 1:500 (Sigma, M1406); chicken anti- NESTIN, 1:2000 (Novus, NB100- 1604); mouse anti- NeuN, 1:500 (Chemicon, MAB377); rabbit anti- NF- M, 1:200 (Millipore, AB1987); mouse anti- NEUROG2, 1:100 (R&D Systems; MAB3314); goat anti- OLIG2, 1:200 (R&D Systems, AF2418); rat anti- RFP, 1:500 (antibodies- online, ABIN334653); rabbit anti- PCP4, 1:500 (Proteintech, 14705–1- AP); rabbit anti- PDGFRB, 1:100 (Cell Signaling, 3169); mouse anti- PSA- NCAM, 1:200 (Chemicon, MAB5324); mouse anti- SATB2, 1:200 (Abcam, ab51502); rabbit anti- SATB2, 1:500 (Abcam, ab34735); rabbit anti- SOX6, 1:500 (Abcam, AB30455); goat anti- SOX10, 1:200 (Santa Cruz, sc- 17342); rabbit anti- SYNAPSIN, 1:500 (Synaptic Systems, 106002); mouse anti- SYNAPTOPHYSIN, 1:500 (Millipore, MAB5258); rabbit anti- TH, 1:250 (Millipore, AB152); rabbit anti- TUBB3 (TUJ1), 1:1000 (Sigma, T2200); mouse anti- TUBB3 (TUJ1), 1:1000 (Biolegend, MMS- 435P), rabbit anti- vGLUT1, 1:500 (Synaptic Systems, 135302); rabbit anti- 2A- peptide, 1:1000 (Millipore, ABS31), rabbit anti- 5HT, 1:3000 (Immunostar, 20080).

    Article Title: Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors
    Article Snippet: The following primary antibodies and dilutions were used: mouse anti-ANK3 (ANKYRIN-G), 1:250 (Santa Cruz, sc-12719); rat anti-BrdU, 1:500 (ACSC, OBT0030); rabbit anti-CSPG4 (NG2), 1:500 (Millipore, AB5320); rabbit anti-CTIP2, 1:500 (Abcam, ab28448); rat anti-CTIP2, 1:250 (Abcam, ab18465); rabbit anti-CUX1, 1:200 (Santa Cruz Biotechnology, sc-13024); rabbit anti-DARPP32, 1:250 (Cell Signaling Technology, 2306S); rabbit anti-FOG2, 1:250 (Santa Cruz Biotechnology, sc-10755); rabbit anti-FOXP2, 1:2000 (Abcam, AB16064); mouse anti-GABA, 1:200 (Sigma, A0310); mouse anti-GFAP, 1:1000 (Sigma, G3893); rabbit anti-GFAP, 1:1000 (Sigma, G9269); chicken anti-GFP, 1:1000 (Invitrogen, A10262); rabbit anti-GFP, 1:1000 (Invitrogen, A11122); mouse anti-HA, 1:1000 (Covance, MMS-101R); mouse anti-ISL1, 1:250 (Novus, H00003670); mouse anti-MAP2, 1:500 (Sigma, M1406); chicken anti-NESTIN, 1:2000 (Novus, NB100-1604); mouse anti-NeuN, 1:500 (Chemicon, MAB377); rabbit anti-NF-M, 1:200 (Millipore, AB1987); mouse anti-NEUROG2, 1:100 (R&D Systems; MAB3314); goat anti-OLIG2, 1:200 (R&D Systems, AF2418); rat anti-RFP, 1:500 (antibodies-online, ABIN334653); rabbit anti-PCP4, 1:500 (Proteintech, 14705-1-AP); rabbit anti-PDGFRB, 1:100 (Cell Signaling, 3169); mouse anti-PSA-NCAM, 1:200 (Chemicon, MAB5324); mouse anti-SATB2, 1:200 (Abcam, ab51502); rabbit anti-SATB2, 1:500 (Abcam, ab34735); rabbit anti-SOX6, 1:500 (Abcam, AB30455); goat anti-SOX10; 1:200 (Santa Cruz, sc-17342); rabbit anti-SYNAPSIN, 1:500 (Synaptic Systems, 106002); mouse anti-SYNAPTOPHYSIN, 1:500 (Millipore, MAB5258); rabbit anti-TH, 1:250 (Millipore, AB152); rabbit anti-TUBB3 (Tuj1), 1:1000 (Sigma, T2200); mouse anti-TUBB3 (Tuj1), 1:1000 (Biolegend, MMS-435P), rabbit anti-vGLUT1, 1:500 (Synaptic Systems, 135302); rabbit anti-2A-peptide, 1:1000 (Millipore, ABS31), rabbit anti-5HT, 1:3000 (Immunostar, 20080).



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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Image Search Results


    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Journal: bioRxiv

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    doi: 10.64898/2026.05.07.723626

    Figure Lengend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Article Snippet: At 54 hpf larvae were fixed in 2% PFA for 2 hours at room temperature, washed extensively in PBS with 0.3% TritonX-100 (PBX), incubated in blocking buffer (BB: PBX, 0.5% BSA, 10% goat serum) for 2 hours at room temperature, incubated overnight at 4°C in BB containing 1/50 dilution of mouse anti-ISL1/2 antibody (Iowa Developmental Studies Hybridoma Bank #39.4D5), washed extensively with PBX, incubated at room temperature for 2 hours in BB with a 1/200 dilution of goat anti-mouse IgG2b Alexa Fluor568 (Invitrogen #A-21144), washed extensively with PBX, and mounted in 1:1 PBS:glycerol for imaging on a Zeiss LSM800 confocal microscope.

    Techniques: Fluorescence, Control, Staining